مرحبا جميعا ...
حوار مع بكتيريا
ﻣﻌﻨﺎ ﻳﻮﻡ ﺿﻴﻒ ﻋﻤﻼﻕ ﻣﻦ ﻋﻤﻼﻗﺔ Bacteria ﻫﻮ ﻏﻨﻲ ﻋﻦ ﺗﻌﺮﻳﻒ ﻣﻌﻨﺎ ﻓﻲ ﺍﺳﺘﺪﻳﻮ * Staphylococcus*
ﺃﻫﻼ ﺑﻜﻢ ﺟﻤﻴﻌﺎ ﺳﻌﻴﺪ ﺟﺪﺍ ﺃﻥ ﺃﻛﻮﻥ ﺿﻤﻦ ﻫﺬﺍ ﻣﺴﻠﺴﻞ
ﻗﺒﻞ ﺧﻮﺽ ﻓﻲ ﺣﺪﻳﺚ ﻣﻌﻚ ﻋﺮﻓﻨﺎ ﻋﻦ ﺃﻳﻦ ﺗﺮﻋﺮﺕ ﻭ ﺍﻛﺘﺸﻔﺖ؟ ؟
ﺑﺪﺍﻳﺔ 1880 ﻛﺎﻧﺖ ﺍﻟﺒﺪﺍﻳﺔ ﺣﻴﺚ ﺗﻢ ﺍﻛﺘﺸﺎﻓﻲ ﻣﻦ ﻗﺒﻞ ﻋﺎﻟﻢ ﺟﺮﺍﺡ ﻓﻲ ﺍﺑﺮﺩﻳﻦ، ﺍﺳﻜﺘﻠﻨﺪﺍ .
ﻃﺒﻌﺎً ﺃﻧﺘﻤﻲ ﺇﻟﻰ ﻋﺎﺋﻠﺔ ﻭ ﻧﻮﻉ G.positive ﻭﺃﻳﻀﺎ ﻳﻄﻠﻖ ﻋﻠﻰ ﺍﺳﻤﻲ ﺍﺧﺘﺼﺎﺭ staph ﻭ ﺷﻜﻠﻲ
Grape like clusters .
ﻭﻳﻄﻠﻖ ﻋﻠﻰ ﺷﻜﻞ Cocci
ﺳﻴﺪ staph ﺃﻳﻦ ﻣﻜﺎﻥ ﺗﻮﺍﺟﺪ ؟؟
ﻋﺎﺩﺓ ﺃﻛﻮﻥ ﻋﻠﻰ
Surface of body &Skin& Nose ,,,
ﻳﺎﻋﻢ Staph ﺗﺼﻨﻒ ﻣﻦ
Pathogenic Or Commensals
ﺻﺮﺍﺣﺔ ﻣﺎ ﺭﺍﺡ ﺍﻛﺬﺏ ﻋﻠﻴﻚ ﻟﺪﻱ ﺍﻭﻻﺩ ﺑﻌﻀﻬﻢ pathogenic ﻭ ﺑﻌﺾ ﺁﺧﺮ ﺑﺎﺭ ﻭ ( ﺻﻤﺼﻮﻡ : ﻣﺆﺩﺏ ) Commensals ﻭﻫﻢ ﺃﻏﻠﺒﻴﺔ
ﺩﺍﻳﻢ ﻣﺎ ﻧﺴﻤﻊ ﻋﻦ ﺍﺳﻢ ﺁﺧﺮ ﻣﺸﺎﺑﻪ ﻟﻚ ﻫﻮ strpto ﺃﻥ ﺷﺎﺀ ﺍﻟﻠﻪ ﺑﻴﻜﻮﻥ ﺿﻴﻒ ﺍﻟﺤﻠﻘﺔ ﺍﻟﻘﺎﺩﻣﺔ
ﻛﻴﻒ ﺍﻓﺮﻕ ﺑﻴﻦ Strpto & ﺣﻀﺮﺗﻚ Staph
ﺗﻔﺮﻳﻖ ﺳﻬﻞ ﺟﺪﺍً ﺑﺎﺳﺘﺨﺪﺍﻡ ﻓﺤﺺ *Catalase* ﺟﻤﻴﻊ Staph ﻳﻌﻄﻲ Positive
ﺃﻣﺎ Strpto ﻳﻌﻄﻴﻚ Negative
ﻫﺬﺍ ﻓﺤﺺ ﻟﻲ ﻳﻌﺘﺒﺮ ﻓﺤﺺ ﺗﻔﺮﻳﻘﻲ
ﺳﻴﺪ staph ﻣﺎﻫﻲ ﺍﻟﺨﺼﺎﺋﺺ ﺍﻟﺘﻲ ﺗﺘﻤﻴﺰ ﺑﻬﺎ ؟؟
ﻛﻤﺎ ﻗﻠﺖ ﺁﻧﻔﺎ ﺃﻧﻨﻲ
G.positive Cocci
Aerobically on ordinary media.
non sporing
non capsulated
non motile
Temperature 37C°
ﻣﺎﺷﺎﺀ ﺍﻟﻠﻪ ﺣﺎﺝ Staph
ﻣﻦ ﻫﻮﻻﺀ ﺍﻭﻻﺩ ﻟﻲ ﻣﻌﻚ ﻓﻲ ﺍﺳﺘﺪﻳﻮ
ﺫﻳﻼ ﺍﻭﻻﺩﻱ ﻭ ﺍﻟﺒﻘﻴﺔ ﻓﻲ ﺑﻴﺖ
ﻧﺘﻌﺮﻑ ﻋﻠﻰ ﺍﻭﻻﺩ Staph
ﺛﻼﺛﺔ ﺍﻭﻻﺩﻱ
ﺍﻭﻝ ﺍﺳﻤﻪ aureus
ﺛﺎﻧﻲ ﺍﺳﻤﻪ epidermidis
ﺛﺎﻟﺚ ﺍﺳﻤﻪ saprophyticus
ﺃﻫﻼ ﻭﺳﻬﻼ ﺑﻜﻢ ﺍﻭﻛﻲ ﻧﺘﻌﺮﻑ ﺃﻛﺜﺮ aureus
ﺃﻫﻼ ﻭﺳﻬﻼ ﻟﻲ ﺷﺮﻑ ﺃﻥ ﺗﻌﺮﻓﻮﻧﻲ
ﻋﺮﻓﻨﺎ ﻋﻨﻚ ﺑﺎﺧﺘﺼﺎﺭ؟ ؟
ﺃﻭﻝ ﺣﺎﺟﺔ ﻟﻜﻲ ﺗﻔﺮﻕ ﺑﻴﻨﻲ ﻭ ﺑﻴﻦ ﺍﺧﻮﺗﻲ ﺑﻔﺤﺺ *coagulase*
ﻧﻌﻄﻲ ﻓﻲ ﻫﺬﺍ ﻓﺤﺺ Positive
ﺍﻣﺎ ﺃﺧﻮﺍﺗﻲ ﺑﻘﻴﺔ ﻳﻌﻄﻮﻥ Negative
ﻭﺃﻳﻀﺎ ﻫﻢ ﻏﺎﻟﺒﺎ Commensals
ﺃﻣﺎ ﺃﻧﺎ main pathogen responsible for pyogenic infection
ﺳﻴﺪ aureus ﺣﺪﺛﻨﻲ ﻋﻦ
Identification ﺗﺒﻌﻚ
ﺑﺪﺍﻳﺔ ﺑﺈﻣﻜﺎﻧﻚ ﺟﻤﻊ ﻋﻴﻨﺎﺕ ﻣﻦ swap , blood, stool, suptum, pus , ,
ﻣﺎﺫﺍ ﻋﻦ culture
ﻋﺎﺩﺓً ﻓﻲ nutrient agar ﻭ ﺗﻜﻮﻥ ﻣﺴﺘﻌﻤﺮﺍﺕ golden colonies
ﺑﺈﻣﻜﺎﻧﻚ ﺃﻳﻀﺎً ﺗﺰﺭﻳﻊ ﻓﻲ
Blood agar ﺗﻜﻮﻥ
B- hemolytic
ﻣﺎﻫﻲ selective media ﺗﺒﻌﻚ؟ ؟؟
ﻫﻲ . Mannitol Salt agar
ﻣﺎﺷﺎﺀ ﺍﻟﻠﻪ ﻳﺒﺪﻭﺍ ﺃﻥ ﻭﻗﺖ ﺑﺮﻧﺎﻣﺞ ﻗﺪ ﺷﺎﺭﻑ ﻋﻠﻰ ﺍﻟﺮﺣﻴﻞ ﺷﺮﻓﺘﻮﻧﺎ ﻋﺎﺋﻠﺔ Staph
ﻟﻨﺎ ﺍﻟﺸﺮﻑ ﺃﻛﺜﺮ ﻭﺷﻜﺮﻧﺎ ﻟﻜﻢ...
رد مع اقتباس
المشاركة الأصلية كتبت بواسطة رجل كهل
